biotechnologies - master biotechnologies parcours structural and functional biochemistry par Université Toulouse III - Paul Sabatier - Mission Formation Continue et Apprentissage

Syllabus du M1 BT Syllabus du M2 BT
- SFB

2nd year of master Structural and Fuctional Biochemistry Our objective is to give you a solid, thorough training in the art of thinking: clear reasoning, critical analysis, deciphering complex subjects, and techniques such as conveying your thoughts accurately.Our goal is to produce graduates with detailed knowledge of molecular mechanisms in Biology applied to pharmacology, structural biology, cellular biophysics or biotechnology.Graduating students will thereby gain an unique perspective on biological problems through their contribution to scientific research projects. [u]Technological workshop[/u]Students will follow lectures and practicals on cutting
- edge techniques. A list of 5 categories of techniques is proposed: molecular biology, structural biology of macromolecules, metabolomics, proteomics and biophysics. Student will be asked to choose 3 of these and will receive 50 hours of practical and lectures of each. This will allow students to adapt their formation to their professional goals.Students will receive theoretical and technical training on most commonly used in the following fields:
- Molecular biology: study of the different methods used in molecular biology, This module willbe mainly based on the analysis of "Matherial and Methods" of recently published papers, Student will be asked to find alternatives methods and to discuss results.
- Biophysics: super
- resolution fluorescence microscopy, Small
- angle X
- ray scattering, calorimetry, circular dichroism, electron microscopy, atomic force microscopy (AFM), analytic ultracentrifugation.
- Proteomics: Ionization modes (ESI, MALDI), scanning modes (TOF, q
- TOF, Orbitrap...), strategies of analysis (bottom
- up, top
- down/middle
- down), study of postraductional modifications of proteins and interactomes using mass spectrometry, informatics data treatment.
- Structural biology of macromolecules: 2D and 3D NMR; X
- ray crystallography, electronic microscopy, molecular modelization.
- Metabolomics: This course covers modern research on metabolism and is orientated to systems approaches in metabolic research. As well as acquiring an in
- depth understanding of modern principles in metabolism, students will develop skills in methods (metabolomics, fluxomics) to investigate metabolism at the system level [u]Thematic Workshop[/u]This teaching unit will depend on the professional project of each student:
- For students aiming at pursuing their studies by a PhD degree, students will have to create a thesis project starting from 3 to 4 published papers on a specific theme. These themes will be related to the main topics of the master (Structural Biology, Proteomics, Biophysics, Metabolomics, Molecular Biology...) and will change every year. Each student will have to create a thesis project under the supervision of teachers and researches specialised in the domain. The thesis project will be evaluated according the rules of the Doctoral School of Toulouse ""BSB"" (10 minutes of presentation and 15 minutes of que